Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures
نویسندگان
چکیده
منابع مشابه
Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells
The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have anal...
متن کاملAmniotic fluid cell cultures.
In an 18-month period, 249 samples of amniotic fluid were used experimentally in attempts to enhance success in cell cultures. None ofthe experimental situations proved entirely satisfactory in this aim. Emphasis is laid on the quality ofserum used in culture. Particularly satisfactory serum should be retained for use in amniotic fluid cell cultures which are dependent on unknown growth stimula...
متن کاملUmbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells
OBJECTIVES The diverse clinical applications for human mesenchymal stem cells (hMSCs) in cellular therapy and regenerative medicine warrant increased focus on developing adequate culture supplements devoid of animal-derived products. In the present study, we have investigated the feasibility of umbilical cord blood-platelet lysate (UCB-PL) as a standard substitute for fetal bovine serum (FBS) a...
متن کاملPreparation of pooled human platelet lysate (pHPL) as an efficient supplement for animal serum-free human stem cell cultures.
Platelet derived growth factors have been shown to stimulate cell proliferation efficiently in vivo(1,2) and in vitro. This effect has been reported for mesenchymal stromal cells (MSCs), fibroblasts and endothelial colony-forming cells with platelets activated by thrombin(3-5) or lysed by freeze/thaw cycles(6-14) before the platelet releasate is added to the cell culture medium. The trophic eff...
متن کاملSenescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy
Human amniotic-fluid-derived mesenchymal stem cells (AF-MSCs) are interesting for their multilineage differentiation potential and wide range of therapeutic applications due to the ease of culture expansion. However, MSCs undergo replicative senescence. So far, the molecular mechanisms that underlie fetal diseases and cell senescence are still poorly understood. Here, we analyzed senescence-ass...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2019
ISSN: 2296-4185
DOI: 10.3389/fbioe.2019.00338